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KMID : 0545120090190101153
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 10 p.1153 ~ p.1160
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Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis
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Han Woo-Cheul
Byun Sun-Ho
Kim Mi-Hyun
Sohn Eun-Hwa
Lim Jung-Dae
Um Byung-Hun
Kim Chul-Ho
Kang Soon-Ah
Jang Ki-Hyo
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Abstract
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Lactosucrose (4G-β-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23oC, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to be O-β-D-galactopyranosyl-(1→4)-O-β-D-glucopyranosyl- (1→2)-β-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as s result.
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KEYWORD
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glucose oxidase, lactosucrose, levansucrase, Zymomonas mobilis
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